How the notebook worksEvery page answers two questions: what kind of thinking it keeps, and how far along that thinking is.What it keepsNotesmy thoughts in fluxTopicA gathering point. It collects the notes underneath it, and sometimes nothing is written in the middle.Minethis oneMy ideas, at every stage of thought.InspiredNot fully, or mostly, original. It says whose idea it started as.StubNot enough to say in public yet. Here for the links in and out.How far alongSteepingThin and early. Written down so I can find out what I think of it.BrewingHas a shape, still moving. Most of the notebook lives here.PouredFinished, which is not the same as long. Five words can be poured.Mix kind and stage on the benchEdited
Fixing cells with Formaldehyde
Fixing cells with formaldehyde for IF staining can lead to autofluorescence. Note that this can effect the sensitivity and specificity of assays.
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The most common reason for increased autofluorescence is pH drift of the formaldehyde. It is important that correct pH is established in fresh formaldehyde and that pH is monitored as the fixative solution ages.
Fixing Cells with Formaldehyde and Increased Autofluorescence | Flow Cytometry
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